A knowledge transfer initiative resulted in the creation of an open access video from the Yimon Aye lab (EPFL) how to monitor on-target signaling responses in larval zebrafish using Z-REX method.

On June 2, 2023, as part of its closure activities, the former NCCR Chemical Biology initiated a call for sponsorship to showcase techniques developed in an NCCR lab through filming. The purpose of this was to have the filmed technique published in JoVE, a global leader in producing and providing science videos with the mission to enhance scientific research and education. The result of this initiative is published today featuring a tool for scientists wishing to monitor on-target signaling responses using Z-REX method.

Zebrafish targeting reactive electrophiles and oxidants (Z-REX) is a chemical biology-based method for the investigation of reactive small-molecule signaling. This technique can be applied to live fish of different developmental stages and Z-REX can link a specific ligand target engagement with a specific phenotype with high spatiotemporal precision. Here, the authors from the Aye lab (EPFL) show how to couple standard assays in zebrafish with Z-REX and monitor on-target signaling responses in larval zebrafish.

View the video

• Kuan-Ting Huang, Phillippe Ly, Jesse R. Poganik, Saba Parvez, Marcus J. C. Long, Yimon Aye, Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites, Jove (2023), Open access. [More Information]